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Recent publications from the Ensign laboratory:

Wampler, D. A. and Ensign, S. A. 2005 " Photoheterotrophic metabolism of Acrylamide by a Newly Isolated Strain of Rhodopseudomonas palustris" Appl. Environ. Microbiol. 71, 5850-5857.

Boyd, J. M. and Ensign, S. A. 2005 “Evidence for a Metal-Thiolate Intermediate in Alkyl Group Transfer from Epoxypropane to Coenzyme M and Cooperative Metal Ion Binding in Epoxyalkane:CoM Transferase” Biochemistry, 44, 13151-13162.

Boyd, J. M. and Ensign, S. A. 2005 “ATP-dependent Enolization of Acetone by Acetone Carboxylase from Rhodobacter capsulatus Biochemistry, 44, 8543-8553.

Boyd, J. M., Ellsworth, H. and Ensign, S. A. 2004 “Bacterial acetone carboxylase is a manganese-dependent metalloenzyme” The Journal of Biological Chemistry, 279, 46644-46651.

Clark, D. D., Boyd, J.M., and Ensign, S. A 2004 “The Stereoselectivity and Catalytic Properties of Xanthobacter autotrophicus 2-[(R)-2-Hydroxypropylthio]ethanesulfonate Dehydrogenase Are Controlled by Interactions between C-Terminal Arginine Residues and the Sulfonate of Coenzyme M” Biochemistry, 43, 6763-6771.

Ensign, S. A. and Allen, J. R. 2003 “Aliphatic Epoxide Carboxylation” Annual Review of Biochemistry, 72, 55-76.

Nocek, B., Jang, S. B., Jeong, M. S., Clark, D. D., Ensign, S. A., Peters, J. W†. 2002 “Structural basis for CO2 fixation by a novel member of the disulfide oxidoreductase family of enzymes: 2-ketopropyl-Coenzyme M Oxidoreductase/ Carboxylase” Biochemistry, 41, 12907-12913.

Sluis Miriam. K., Larsen, Rachel A.., Krum, Jonathan. G., Anderson, Ruth., Metcalf, William. W. and Ensign, Scott A. 2002 "Biochemical, molecular and genetic characterization of the ATP-dependent acetone carboxylases of Rhodobacter capsulatus and Xanthobacter autotrophicus strain Py2". The Journal of Bacteriology, 184, 2969-2977.

Krum, Jonathan. G., Ellsworth, Heather, Sargeant, Ryan., Rich, Greg., and Ensign, Scott. A†. 2002 “Kinetic and Microcalorimetric Analysis of Substrate and Cofactor Interactions in Epoxyalkane:CoM Transferase: a Zn-Dependent Epoxidase”. Biochemistry, 41, 5005-5014.

Clark, Daniel. D. and Ensign, S. A. 2002 "Characterization of the 2-[(R)-2-hydroxypropylthio] ethanesulfonate Dehydrogenase from Xanthobacter strain Py2: Product Inhibition, pH-Dependence of Kinetic Parameters, Site-Directed Mutagenesis, Rapid-Equilibrium Inhibition, and Chemical Modification". Biochemistry, 41, 2727-2740.

Ensign, Scott. A. 2001 “Microbial Metabolism of Aliphatic Alkenes”, Biochemistry, 40, 5845-5853.

Krum, Jonathan. G. and Ensign, Scott. A. 2001 "Evidence that a linear megaplasmid in Xanthobacter strain Py2 encodes the enzymes of aliphatic alkene and epoxide metabolism and Coenzyme M (2-mercaptoethanesulfonate) biosynthesis" the Journal of Bacteriology, 183, 2172-2177

Krum, Jonathan G., and Ensign, Scott A. 2000 "Heterologous expression of bacterial epoxyalkane:coenzyme M transferase and inducible coenzyme M biosynthesis in Xanthobacter strain Py2 and Rhodococcus rhodochrous B276." the Journal of Bacteriology, 182: 2629-2634..

Clark, Daniel D., Allen, Jeffrey R., and Ensign, Scott A. 2000 "Characterization of Five Catalytic Activities Associated with the NADPH:2-Ketopropyl-coenzyme M [2-(2-Ketopropylthio)ethanesulfonate] Oxidoreductase/Carboxylase of the Xanthobacter Strain Py2 Epoxide Carboxylase System." Biochemistry, 39, 1294-1304.

Allen, Jeffrey R., Clark, Daniel D., Krum, Jonathan G. , and Ensign, Scott A†. 1999 "A role for coenzyme M in a bacterial pathway of aliphatic epoxide carboxylation" the Proceedings of the National Academy of Sciences, 96:8432-8437..

Clark, Daniel D. and Ensign, Scott A. 1999 "Evidence for an inducible nucleotide-dependent acetone carboxylase in Rhodococcus rhodochrous strain B276" J. Bacteriol., 181, 2752-2758.

Allen, Jeffrey R. and Ensign, Scott A†. 1999 " Two Short-Chain Dehydrogenases Confer Stereoselectivity for Enantiomers of Epoxypropane in the Multiprotein Epoxide Carboxylating Systems of Xanthobacter strain Py2 and Nocardia corallina B276." Biochemistry, 38, 247-256.

Allen, Jeffrey R. and Ensign, Scott A†. 1998 "Identification and characterization of epoxide carboxylase activity in cell extracts of Nocardia corallina strain B276" the Journal of Bacteriology, 180, 2072-2078.
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Ensign, Scott A.†, Small, Frederick J., Allen, Jeffrey R., and Sluis, Miriam K. 1998 “New Roles for CO2 in the Microbial Metabolism of Aliphatic Epoxides and Ketones" Archives of Microbiology, 169, 179-187.

Allen, Jeffrey R. and Ensign, Scott A†. 1997 "Purification to homogeneity and reconstitution of the individual components of the epoxide carboxylase multiprotein enzyme complex from Xanthobacter strain Py2." the Journal of Biological Chemistry 272, 32121-32128.

Holz, Richard C†., Small, Frederick J., and Ensign, Scott A†. 1997 "1H NMR Investigation of the [2Fe-2S]1- Containing "Rieske-Type" Protein from Xanthobacter Strain Py2" Biochemistry, 36, 14690-14696.

Small, Frederick J. and Ensign, Scott A† 1997 "Alkene Monooxygenase from Xanthobacter strain Py2: Purification and Characterization of A Four-Component System Central to the Bacterial Metabolism of Aliphatic Alkenes" the Journal of Biological Chemistry 272, 24913-24920.

Sluis Miriam K. and Ensign, Scott A†. 1997 "Purification and Characterization of Acetone Carboxylase from Xanthobacter strain Py2" The Proceedings of the National Academy of Sciences, 94, 8456-8461.

Allen, Jeffrey R. and Ensign, Scott A†. 1997 "Characterization of Three Protein Components Required for Functional Reconstitution of the Epoxide Carboxylase Multienzyme Complex from Xanthobacter strain Py2" J. Bacteriol., 179, 3110-3115.

Sluis Miriam K., Small, Frederick J., Allen, Jeffrey R. and Ensign, Scott A†. 1996 "Involvement of an ATP-Dependent Carboxylase in a CO2-Dependent Pathway of Acetone Metabolism by Xanthobacter strain Py2" J. Bacteriol. 178, 4020-4026.

Allen, Jeffrey R. and Ensign, Scott A†. 1996 "Carboxylation of Epoxides to ß-Keto Acids in Cell Extracts of Xanthobacter Strain Py2" J. Bacteriol. 178, 1469-1472.

Ensign, Scott A†. 1996 "Aliphatic and chlorinated alkenes and epoxides as inducers of alkene monooxygenase and epoxidase activities in Xanthobacter strain Py2" Appl. Environ. Microbiol. 62, 61-66.

Small, Frederick J. and Ensign, Scott A†. 1995 "Carbon Dioxide Fixation in the Metabolism of Propylene and Propylene oxide by Xanthobacter strain Py2" J. Bacteriol. 177, 6170-6175.

Seefeldt, Lance C†., Rasche, Madeline E., and Ensign, Scott A. 1995 "Carbonyl Sulfide and Carbon Dioxide as New Substrates and Carbon Disulfide as a New Inhibitor of Nitrogenase." Biochemistry, 34, 5382-5389.

Ensign, Scott A†. 1995 "Reactivity of Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum with Carbon Dioxide, Carbonyl Sulfide, and Carbon Disulfide." Biochemistry, 34, 5372-5381.

Small, Frederick J., Tilley, James K. and Ensign, Scott A†. 1995 "Characterization of a New Pathway for Epichlorohydrin Degradation by Whole Cells of Xanthobacter strain Py2." Appl. Environ. Microbiol. 61, 1507-1513.

Seefeldt, Lance C†. and Ensign, Scott A. 1994 "A Continuous Spectrophotometric Activity Assay for Nitrogenase Using the reductant Titanium (III) Citrate." Analytical Biochemistry, 221, 379-386.

 



© 2004 Scott A. Ensign

Department of Chemistry and Biochemistry
0300 Old Main Hill
Logan, UT 84322
(435) 797-3969
ensigns@cc.usu.edu